Target
Human SLC18A1 / VMAT1
Synonyms
SLC18A1 | CGAT | Vesicular amine transporter 1 | VMAT1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
A 20 aa peptide sequence near the C-Terminus, cytoplasmic region of human VMAT1.
Specificity
Recognizes human Vesicular Monoamine Transporter 1 (VMAT1). Species sequence Homology: rat: 63%. No significant sequence homology with VMAT2 or other transporters.
Applications
- Western blot (1 - 10 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml using ECL. Detects a major band at ~55 kD in postnuclear supernatants of CHO transfected with VMAT1 and not in wild type cells. Some additional bands, both higher and lower MW, were also detected and remain unaffected by the inclusion of protease inhibitors. ELISA: 0.5-1 ug/ml. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Presentation
100 mM Tris, pH 7.5, 0.05% Sodium Azide, 40% Glycerol, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee