VGLUT1 antibody LS-C16706 is an unconjugated rabbit polyclonal antibody to human VGLUT1 (SLC17A1). Validated for ELISA and WB.
Human SLC17A1 / VGLUT1
(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
Human NPT1 is 465/467aa (chromosome 6) and mouse and rat 465 aa proteins. A 14 AA synthetic peptide within the cytoplasmic, N-terminus of Human NPT1/NaPi-4 was synthesized, conjugated to KLH.
Peptide is 78% conserved in rat and 71% in mouse Npt1. No significant sequence homology is observed with other Pi-transporters (Type-II-IV) transporters. Antibody cross-reactivity in various species is not established.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, pH 7.4, 0.1% BSA
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.