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Anti-SLC12A4 / KCC1 Antibody (aa997-1007) IHC-plus™ LS-B2377

Note: This antibody replaces LS-C55024

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SLC12A4 / KCC1 Antibodies

Anti-SLC12A4 / KCC1 Antibody (aa997-1007) IHC-plus™ LS-B2377
Goat Polyclonal to Human SLC12A4 / KCC1
Human, Mouse, Rat, Bat, Dog, Hamster
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SLC12A4 / KCC1 Antibody LS-C333065
Rabbit Polyclonal (IgG) to Human SLC12A4 / KCC1
Human, Mouse, Rat
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human SLC12A4 / KCC1
Human, Mouse, Rat, Bat, Dog, Hamster
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SLC12A4 / KCC1
Goat
Human, Mouse, Rat, Bat, Dog, Hamster (tested or 100% immunogen sequence identity)
Monkey, Bovine, Horse, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:4000)

Specificity and Use

SLC12A4 / KCC1 antibody was raised against synthetic peptide C-DPSHAPDNFRE from an internal region of human SLC12A4 (NP_005063.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Dog, Bat (100%); Marmoset, Panda, Bovine, Rabbit, Horse (91%).
LS-E26489 - Lyophilized - 100 µg - $145.00
aa997-1007
Human SLC12A4.
Immunohistochemistry: LS-B2377 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2377 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SLC12A4 / KCC1

Q9UP95 NM_005072 NP_005063.1

SLC12A4 Antibody, Erythroid K-Cl cotransporter 1 Antibody, HKCC1 Antibody, KCC1 Antibody, K-Cl cotransporter Antibody, Erythroid K:Cl cotransporter Antibody

Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl- exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity.

Immunohistochemistry

Immunohistochemistry
Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/9/2016

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