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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLC12A4 / KCC1 Antibody (aa997‑1007) IHC‑plus™ LS‑B2377 Best Seller
Note: This antibody replaces LS-C55024
KCC1 antibody LS-B2377 is an unconjugated goat polyclonal antibody to KCC1 (SLC12A4) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B2377-50
50 µg (0.5 mg/ml)
$425

Popular SLC12A4 / KCC1 Products

Immunohistochemistry of paraffin-embedded human placenta tissue using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Immunohistochemistry of Human colon cancer using SLC12A4 Polyclonal Antibody at dilution of 1:30.
Species: Human, Mouse, Rat
Applications: IHC, ELISA
Immunohistochemistry of Human brain using SLC12A4 Polyclonal Antibody at dilution of 1:30.
Species: Human, Mouse, Rat
Applications: IHC, ELISA
Western Blot analysis of SLC12A4 expression in transfected 293T cell line by SLC12A4 monoclonal antibody (M01), clone 1H6.Lane 1: SLC12A4 transfected lysate(120.6 KDa).Lane 2: Non-transfected lysate.
Species: Human
Applications: Western blot, ELISA

Product Description

KCC1 antibody LS-B2377 is an unconjugated goat polyclonal antibody to KCC1 (SLC12A4) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About SLC12A4 / KCC1
Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl- exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity. Q9UP95 NM_005072 NP_005063.1

SLC12A4 Antibody, Erythroid K-Cl cotransporter 1 Antibody, HKCC1 Antibody, KCC1 Antibody, K-Cl cotransporter Antibody, Erythroid K:Cl cotransporter Antibody


Specifications

Target
Human SLC12A4 / KCC1
Host
Goat
Reactivity
Human, Mouse, Rat, Bat, Dog, Hamster (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SLC12A4 / KCC1 antibody was raised against synthetic peptide C-DPSHAPDNFRE from an internal region of human SLC12A4 (NP_005063.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Dog, Bat (100%); Marmoset, Panda, Bovine, Rabbit, Horse (91%).
Blocking Peptide
LS-E26489 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2377. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa997-1007
Specificity
Human SLC12A4.
Usage
Immunohistochemistry: LS-B2377 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2377 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

Publications (1)

1
The Zn(2+)-sensing receptor, ZnR/GPR39, upregulates colonocytic Cl(-) absorption, via basolateral KCC1, and reduces fluid loss. Sunuwar L, Asraf H, Donowitz M, Sekler I, Hershfinkel M. Biochimica et biophysica acta. 2017 1863:947-960. (WB; Human) [Full Text Article] [PubMed:28093242] [PMC:PMC5557417]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.
Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.

Western blot

Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.
Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.

Western blot

Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.
Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.

Western blot

Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.
Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2377 concentration 5 ug/ml.

Western blot

Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 9/18/2018

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