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Anti-SLC12A1 / NKCC2 Antibody LS-C150446

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SLC12A1 / NKCC2 Antibodies

Anti-SLC12A1 / NKCC2 Antibody (aa351-400) LS-C40323
Rabbit Polyclonal (IgG) to Human SLC12A1 / NKCC2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
IHC, Western blot
Unconjugated
Western blot Image
Anti-SLC12A1 / NKCC2 Antibody (aa43-57) IHC-plus™ LS-B5131
Goat Polyclonal to Human SLC12A1 / NKCC2
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-SLC12A1 / NKCC2 Antibody (aa125-139) LS-C313275
Rabbit Polyclonal (IgG) to Human SLC12A1 / NKCC2
Human, Monkey, Mouse, Rat, Guinea pig, Pig, Rabbit
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Rat SLC12A1 / NKCC2
Rat, Human, Mouse
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Rat SLC12A1 / NKCC2
Rabbit
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Atto 390, Atto 488, Atto 565, Atto 594, Atto 633, Atto 655, Atto 680, Atto 700, AP, APC, Biotin, FITC, PE, Atto 594, PerCP, RPE, SA.
Affinity purified
Unmodified

Applications

  • Western blot (1:1000)
  • (applications tested for the base form of this product only)

Specificity and Use

SLC12A1 / NKCC2 antibody was raised against synthetic peptide mapping to a segment (amino acids 33-55, NP_001073158) of the N-terminal tail of rat NKCC2 (antibody desgination L224)
Detects ~160kDa. Cross-reactivity with human is weak.

Packaging

PBS, 0.09% sodium azide, 50% glycerol.
Store at -20°C for up to one year.
For research use only.

Publications (1)

1
Whole-exome resequencing reveals recessive mutations in TRAP1 in individuals with CAKUT and VACTERL association. Saisawat P, Kohl S, Hilger AC, Hwang DY, Yung Gee H, Dworschak GC, Tasic V, Pennimpede T, Natarajan S, Sperry E, Matassa DS, Staji[Character 107] N, Bogdanovic R, de Blaauw I, Marcelis CL, Wijers CH, Bartels E, Schmiedeke E, Schmidt D, M[Character e4]rzheuser S, Grasshoff-Derr S, Holland-Cunz S, Ludwig M, N[Character f6]then MM, Draaken M, Brosens E, Heij H, Tibboel D, Herrmann BG, Solomon BD, de Klein A, van Rooij IA, Esposito F, Reutter HM, Hildebrandt F. Kidney international. 2014 Jun;85:1310-7. [Full Text Article] [PubMed:24152966] [PMC:PMC3997628]

About SLC12A1 / NKCC2

Q13621 NM_000338 NP_000329.2

SLC12A1 Antibody, BSC1 Antibody, MBSC1 Antibody, Na-K-2Cl cotransporter Antibody, NKCC2A variant A Antibody, RBSC Antibody, NKCC2 Antibody

SLC12A1 / NKCC2 is a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene.

Western blot

Western blot
Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution of SLC12A1 / NKCC2 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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