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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLC12A1 / NKCC2 Antibody (aa351‑400) LS‑C40323
NKCC2 antibody LS-C40323 is an unconjugated rabbit polyclonal antibody to NKCC2 (SLC12A1) from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C40323-50
50 µg (1 mg/ml)
Unavailable

Popular SLC12A1 / NKCC2 Products

Antibody
Species: Rat
Applications: IHC, Western blot, ELISA
Anti-SLC12A1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5131 concentration 3.75 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin
Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution of SLC12A1 / NKCC2 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat, Human, Mouse
Applications: Western blot
SLC12A1 / NKCC2 antibody. IHC(P): Rat Kidney Tissue.
Species: Human, Monkey, Mouse, Rat, Guinea pig, Pig, Rabbit
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot

Product Description

NKCC2 antibody LS-C40323 is an unconjugated rabbit polyclonal antibody to NKCC2 (SLC12A1) from human, mouse, rat and other species. Validated for IHC and WB.
About SLC12A1 / NKCC2
SLC12A1 / NKCC2 is a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Q13621 NM_000338 NP_000329.2

SLC12A1 Antibody, BSC1 Antibody, MBSC1 Antibody, Na-K-2Cl cotransporter Antibody, NKCC2A variant A Antibody, RBSC Antibody, NKCC2 Antibody


Specifications

Target
Human SLC12A1 / NKCC2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Guinea pig, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
SLC12A1 / NKCC2 antibody was raised against synthetic peptide located between aa351-400 of human SLC12A1 (Q8IUN5). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Ferret, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Platypus (100%); Gorilla, Elephant, Guinea pig, Zebra finch, Chicken (92%); Turkey (85%).
Epitope
aa351-400
Specificity
Human SLC12A1
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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