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SLC12A1 / NKCC2

solute carrier family 12 (sodium/potassium/chloride transporter), member 1

SLC12A1 / NKCC2 is a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.

Gene Name: solute carrier family 12 (sodium/potassium/chloride transporter), member 1
Family/Subfamily: Transporter , Cation:chloride symporter
Synonyms: SLC12A1, BSC1, MBSC1, Na-K-2Cl cotransporter, NKCC2A variant A, RBSC, NKCC2
Target Sequences: NM_000338 NP_000329.2 Q13621

Publications (2)

1
Critical role of the SPAK protein kinase CCT domain in controlling blood pressure. Zhang J, Siew K, Macartney T, O'Shaughnessy KM, Alessi DR. Human molecular genetics. 2015 24:4545-58. (WB; Human) [Full Text Article] [PubMed:25994507] [PMC:PMC4512625] Related Antibodies: LS-C313275.
2
Whole-exome resequencing reveals recessive mutations in TRAP1 in individuals with CAKUT and VACTERL association. Saisawat P, Kohl S, Hilger AC, Hwang DY, Yung Gee H, Dworschak GC, Tasic V, Pennimpede T, Natarajan S, Sperry E, Matassa DS, Staji[Character 107] N, Bogdanovic R, de Blaauw I, Marcelis CL, Wijers CH, Bartels E, Schmiedeke E, Schmidt D, M[Character e4]rzheuser S, Grasshoff-Derr S, Holland-Cunz S, Ludwig M, N[Character f6]then MM, Draaken M, Brosens E, Heij H, Tibboel D, Herrmann BG, Solomon BD, de Klein A, van Rooij IA, Esposito F, Reutter HM, Hildebrandt F. Kidney international. 2014 Jun;85:1310-7. [Full Text Article] [PubMed:24152966] [PMC:PMC3997628] Related Antibodies: LS-C150446.

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Antibodies (37)
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SLC12A1 / NKCC2 (47)
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SLC12A1 / NKCC2 Antibody - SLC12A1 / NKCC2 antibody. IHC(F): Rat Kidney Tissue.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (aa125-139) Antibody
Mouse, Rat, Human
IHC, IHC-Fr, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
SLC12A1 / NKCC2 Antibody - A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (aa351-400) Antibody
Human
IHC, WB
Unconjugated
100 µl/$424
SLC12A1 / NKCC2 Antibody - A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (aa351-400) (HRP) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Bat, Gibbon, Orangutan, Chimpanzee, Human, Monkey
IHC, WB
HRP Conjugated
100 µl/$469
SLC12A1 / NKCC2 Antibody - A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (aa351-400) (Biotin) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Bat, Gibbon, Orangutan, Chimpanzee, Human, Monkey
IHC, WB
Biotin Conjugated
100 µl/$469
SLC12A1 / NKCC2 Antibody - Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution of SLC12A1 / NKCC2 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SLC12A1 / NKCC2 Rabbit anti-Rat Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µg/$449
SLC12A1 / NKCC2 Antibody - Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution of SLC12A1 / NKCC2 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SLC12A1 / NKCC2 Rabbit anti-Rat Polyclonal (aa33-55) (FITC) Antibody
Mouse, Rat, Human
WB
FITC Conjugated
100 µg/$480
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SLC12A1 / NKCC2 Rabbit anti-Rat Polyclonal (C-Terminus) Antibody
Rat
ELISA, IHC, IHC-Pfa, WB
Unconjugated
100 µg/$832
SLC12A1 / NKCC2 Antibody - SLC12A1 antibody IHC-paraffin: Mouse Kidney Tissue.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (aa52-83) Antibody
Mouse, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
SLC12A1 / NKCC2 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.313 - 20 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Tissue Homogenates
1 Plate/$834
SLC12A1 / NKCC2 Antibody
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100 µg/$575
SLC12A1 / NKCC2 Antibody - Western blot analysis of extracts of 3T3 cells using NKCC2 antibody. The lane on the left was treated with blocking peptide.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
SLC12A1 / NKCC2 Antibody - Western Blot analysis of SLC12A1 expression in transfected 293T cell line by SLC12A1 monoclonal antibody (M03), clone 4H4.Lane 1: SLC12A1 transfected lysate(46.5 KDa).Lane 2: Non-transfected lysate.
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SLC12A1 / NKCC2 Mouse anti-Human Monoclonal (4H4) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$423
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SLC12A1 / NKCC2 Rabbit anti-Rat Polyclonal (C-Terminus) Antibody
Rabbit, Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µl/$801
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (Cytoplasmic Domain) Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µg/$832
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (HRP) Antibody
Mouse, Rat, Human
WB
HRP Conjugated
100 µl/$1,054
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (Biotin) Antibody
Mouse, Rat, Human
WB
Biotin Conjugated
100 µl/$1,054
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (FITC) Antibody
Mouse, Rat, Human
WB
FITC Conjugated
100 µl/$1,054
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (APC) Antibody
Mouse, Rat, Human
WB
APC Conjugated
100 µl/$1,054
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (PE) Antibody
Mouse, Rat, Human
WB
PE Conjugated
100 µl/$1,054
SLC12A1 / NKCC2 Antibody - Western blot analysis of extracts of various cell lines, using SLC12A1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
SLC12A1 / NKCC2 Antibody - Western blot of SLC12A1 / NKCC2 antibody.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (aa814-1099) Antibody
Human
WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
SLC12A1 / NKCC2 Antibody - Western blot of SLC12A1 / NKCC2 antibody.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (aa814-1099) (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$319; 200 µl/$365; 500 µl/$519; 1 ml/$596; 2 ml/$824; 5 ml/$1,348
SLC12A1 / NKCC2 Antibody - Western blot of SLC12A1 / NKCC2 antibody.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal (aa814-1099) (APC, Cy7) Antibody
Human
WB
APC, Cy7 Conjugated
100 µl/$505; 200 µl/$648; 500 µl/$1,152; 1 ml/$1,401; 2 ml/$1,961; 5 ml/$3,676
SLC12A1 / NKCC2 Antibody - Immunochemical staining of human SLC12A1 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.
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SLC12A1 / NKCC2 Rabbit anti-Human Polyclonal Antibody
Human
IHC-P
Unconjugated
100 µl/$412
Select
SLC12A1 / NKCC2 Rabbit anti-Rat Polyclonal (N-Terminus) Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µl/$632
Viewing 1-25 of 47 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).