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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLC11A2 / DMT1 Antibody (4th Cytoplasmic Domain) LS‑C16683
DMT1 antibody LS-C16683 is an unconjugated rabbit polyclonal antibody to rat DMT1 (SLC11A2). Validated for ELISA, IHC and WB.
Antibody
Catalog
Size
Price
LS-C16683-100
100 µg (1 mg/ml)
$755

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Antibody
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Antibody
Species: Rat
Applications: Western blot, ELISA

Product Description

DMT1 antibody LS-C16683 is an unconjugated rabbit polyclonal antibody to rat DMT1 (SLC11A2). Validated for ELISA, IHC and WB.

Specifications

Target
Rat SLC11A2 / DMT1
Synonyms
SLC11A2, Divalent metal transporter 1, Divalent cation transporter 1, DMT-1, NRAMP 2, NRAMP2, Dct-1, DCT1, DMT1
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1 - 10 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SLC11A2 / DMT1 antibody was raised against a 19 aa Peptide sequence located at the predicted cytoplasmic loop # 4 of rat NRAMP2 was synthesized, coupled to KLH.
Epitope
4th Cytoplasmic Domain
Specificity
Recognizes rat Natural Resistance Associated Macrophage Protein (Nramp2). 100% conserved in mouse, 94% in human, 73% in zebra fish NRAMP2. This sequence is common in NRAMP2 with or without IRE isoforms. This antibody will not distinguish between these isoforms.
Usage
Suitable for use in ELISA, Western Blot. Western Blot: 1-10 ug/ml using ECL technique. ELISA: 0.5-1 ug/ml Use 1 ug/ml control peptide/well.
Presentation
PBS, pH 7.5, 0.1% BSA.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About SLC11A2 / DMT1
Solute carrier family 11, member 2 (SLCA2) also known as divalent metal ion transporter 1 (DMT1) imports Fe2+ via a proton-coupled, membrane potential dependent manner. It can also transport a variety of other divalent metal cations including Mn2+, Co2+, Cu2+, and Zn2+ but iron appears to be its most important physiological substrate. P49281 NM_000617 NP_000608.1


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Requested From: United States
Date Requested: 10/22/2018

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