Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Pig, Bovine (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:5 - 1:30)
IHC - Frozen (1:5 - 1:30)
Flow Cytometry (1:5 - 1:30)
Specificity and Use
SLA Class II DQ antibody was raised against porcine Peripheral Blood lymphocytes.
Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2 M boric acid, pH7.0. Has been shown to be cross-reactive with endothelial cells derived from rhesus macaque.
Flow Cytometry: Use 50 ul of the suggested working dilution to label 1 x 10^6 peripheral blood lymphocytes in 100 ul. Method sheets are available on request.