Human SKP2

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Recombinant, full-length human p45SKP2 protein.

Recognizes only human p45SKP2. Cross-reactivity with related endogenous proteins has not been observed.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 2 µg/ml)
• Immunoprecipitation
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B2487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2487 was determined to be 5 ug/ml. Positive control: HeLa, Jurkat and 293T cell lines.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13309 NCBI: NM_005983 NP_005974.2