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SKP1 Antibody LS‑C155251
SKP1 antibody LS-C155251 is an unconjugated rabbit polyclonal antibody to SKP1 from human, rat, bovine and other species. Validated for IHC and WB.
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Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Applications:
Western blot, ELISA
Product Description
SKP1 antibody LS-C155251 is an unconjugated rabbit polyclonal antibody to SKP1 from human, rat, bovine and other species. Validated for IHC and WB.
About SKP1
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling.
P63208
NM_006930
NP_008861.2
SKP1 Antibody, Cyclin A/CDK2-associated p19 Antibody, OCP-2 Antibody, OCP-II Antibody, Organ of Corti protein 2 Antibody, SIII Antibody, TCEB1L Antibody, Organ of Corti protein II Antibody, p19A Antibody, SKP1A Antibody, EMC19 Antibody, OCP2 Antibody, p19skp1 Antibody
Specifications
Target
Human SKP1
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bovine, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Predicted
Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (1:100 - 1:1000)
- Western blot (1:500 - 1:3000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Immunogen
SKP1 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 163 of SKP1 (SwissProt P63208). Percent identity by BLAST analysis: Human, Chicken, Monkey, Rat, Xenopus, Bovine (100%); Zebrafish (99%).
Specificity
Human SKP1
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Immunohistochemistry
IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.
Western blot
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000
Western blot
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000
Immunohistochemistry
IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.
Western blot
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000
Western blot
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000
Immunohistochemistry
IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.
Western blot
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000
Western blot
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000
Immunohistochemistry
IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.
Western blot
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000
Western blot
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000
Requested From:
Date Requested: 6/18/2018
Date Requested: 6/18/2018
