LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
SKP1 Antibody LS‑C155251
SKP1 antibody LS-C155251 is an unconjugated rabbit polyclonal antibody to SKP1 from human, rat, bovine and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C155251-100
100 µl (1 mg/ml)
Unavailable

Popular SKP1 Products

Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot, ELISA
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE SKP1 antibody diluted at 1:1000
Species: Human
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: ELISA

Product Description

SKP1 antibody LS-C155251 is an unconjugated rabbit polyclonal antibody to SKP1 from human, rat, bovine and other species. Validated for IHC and WB.
About SKP1
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. P63208 NM_006930 NP_008861.2

SKP1 Antibody, Cyclin A/CDK2-associated p19 Antibody, OCP-2 Antibody, OCP-II Antibody, Organ of Corti protein 2 Antibody, SIII Antibody, TCEB1L Antibody, Organ of Corti protein II Antibody, p19A Antibody, SKP1A Antibody, EMC19 Antibody, OCP2 Antibody, p19skp1 Antibody


Specifications

Target
Human SKP1
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bovine, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SKP1 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 163 of SKP1 (SwissProt P63208). Percent identity by BLAST analysis: Human, Chicken, Monkey, Rat, Xenopus, Bovine (100%); Zebrafish (99%).
Specificity
Human SKP1
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.
IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000

Western blot

Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.
IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000

Western blot

Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.
IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000

Western blot

Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.
IHC of paraffin-embedded CL1-5 xenograft, using SKP1 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000
Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE SKP1 antibody diluted at 1:3000

Western blot

Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE SKP1 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/18/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number