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Anti-SKP1 Antibody (clone 1H8) LS-C198204

Ordering

Wt. Vol. Conc. Price
100 µg - - Unavailable

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1H8] (IgG2a,k) to Human SKP1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human SKP1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1H8]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

SKP1 antibody was raised against sKP1A (NP_008861, aa53-160 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human SKP1
Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About SKP1

P63208 NM_006930 NP_008861.2

SKP1 Antibody, Cyclin A/CDK2-associated p19 Antibody, OCP-2 Antibody, OCP-II Antibody, Organ of Corti protein 2 Antibody, SIII Antibody, TCEB1L Antibody, Organ of Corti protein II Antibody, p19A Antibody, SKP1A Antibody, EMC19 Antibody, OCP2 Antibody, p19skp1 Antibody

Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to SKP1A on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
SKP1A monoclonal antibody (M01), clone 1H8 Western Blot analysis of SKP1A expression in HeLa.

Western blot

Western blot
Western Blot analysis of SKP1 expression in transfected 293T cell line by SKP1A monoclonal antibody (M01), clone 1H8.Lane 1: SKP1 transfected lysate(18.1 KDa).Lane 2: Non-transfected lysate.

Requested From: 
Date Requested: 4/23/2017

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