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| Catalog Number | Size | Price |
|---|---|---|
| LS-B422-50 | 50 µl | $515 |
IHC‑plus™ Polyclonal Rabbit anti‑Human SKP1 Antibody (aa152‑163, IHC, WB) LS‑B422
IHC‑plus™ Polyclonal Rabbit anti‑Human SKP1 Antibody (aa152‑163, IHC, WB) LS‑B422
Note: This antibody replaces LS-C19095
Antibody:
SKP1 Rabbit anti-Human Polyclonal (aa152-163) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
SKP1 Rabbit anti-Human Polyclonal (aa152-163) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
SKP1 antibody LS-B422 is an unconjugated rabbit polyclonal antibody to human SKP1 (aa152-163). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human SKP1
Synonyms
SKP1 | Cyclin A/CDK2-associated p19 | OCP-2 | OCP-II | Organ of Corti protein 2 | SIII | TCEB1L | Organ of Corti protein II | p19A | SKP1A | EMC19 | OCP2 | p19skp1
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated followed by sterile filtration
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.
Epitope
aa152-163
Specificity
Reacts with human SKP1. Cross reactivity is expected against mouse SKP1 based on a high degree of sequence homology. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known.
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:500 - 1:1000)
- ELISA (1:2000 - 1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B422 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B422 was determined to be 1:500.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SKP1
Validation
Anti-SKP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-SKP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
See More About...
LSBio Ratings
IHC-plus™ SKP1 Antibody (aa152-163) for IHC, WB/Western, ELISA LS-B422 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Molecular cell. 1999 3:535-41.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
