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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIX5 Antibody (aa257‑286) LS‑C161108 Best Seller
SIX5 antibody LS-C161108 is an unconjugated rabbit polyclonal antibody to human SIX5. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C161108-400
400 µl
$305

Popular SIX5 Products

Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

SIX5 antibody LS-C161108 is an unconjugated rabbit polyclonal antibody to human SIX5. Validated for IF, IHC and WB.
About SIX5
Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter (By similarity). Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3. Q8N196 NP_787071.2

SIX5 Antibody, DMAHP Antibody, Homeobox protein SIX5 Antibody, Sine oculis homeobox homolog 5 Antibody, SIX homeobox 5 Antibody, BOR2 Antibody


Specifications

Target
Human SIX5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1904 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C161108. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa257-286
Specificity
This SIX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the Central region of human SIX5.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.
Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.

Western blot

SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).
SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).

Immunohistochemistry

SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.
Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.

Western blot

SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).
SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).

Immunohistochemistry

SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.
Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.

Western blot

SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).
SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).

Immunohistochemistry

SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.
Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.

Western blot

SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).
SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).

Requested From: United States
Date Requested: 9/18/2018

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