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Antibodies > SIX5 > LS-C161108
 
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Catalog Number Size Price
LS-C161108-400 400 µl $393 
SIX5 Antibody - SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIX5 Antibody - Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.
SIX5 Antibody - SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).
SIX5 Antibody - SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIX5 Antibody - Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.
SIX5 Antibody - SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).
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Polyclonal Rabbit anti‑Human SIX5 Antibody (aa257‑286, IHC, IF, WB) LS‑C161108

Polyclonal Rabbit anti‑Human SIX5 Antibody (aa257‑286, IHC, IF, WB) LS‑C161108

Submit Review LSBio Guarantee
Antibody:
SIX5 Rabbit anti-Human Polyclonal (aa257-286) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C161108-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SIX5 Rabbit anti-Human Polyclonal (aa257-286) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SIX5 antibody LS-C161108 is an unconjugated rabbit polyclonal antibody to human SIX5 (aa257-286). Validated for IF, IHC and WB.
Target
Human SIX5
Synonyms
SIX5 | DMAHP | Homeobox protein SIX5 | Sine oculis homeobox homolog 5 | SIX homeobox 5 | BOR2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa257-286
Specificity
This SIX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the Central region of human SIX5.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIX5
Q8N196 NP_787071.2

Publications (0)

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Applications: IHC, Western blot, ELISA
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SIX5 Antibody (aa257‑286, APC) LS‑C240813
Species: Human
Applications: IHC, Western blot, ELISA
SIX5 ELISA Kit
Mouse/Human SIX5 (DNA-Binding ELISA) ELISA Kit - LS-F833
Reactivity: Human, Mouse
Range:
SIX5 Protein - Purified recombinant protein SIX5 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Human SIX5 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G72550
Source: Human
Tag: Myc-DDK (Flag)
SIX5 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SIX5 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G92851
Source: Human
Tag: Myc-DDK (Flag)
See all SIX5 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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