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Anti-SIX5 Antibody (aa257-286) LS-C161108

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SIX5 Antibodies

Anti-SIX5 Antibody (aa257-286, FITC) LS-C240816
Rabbit Polyclonal (IgG) to Human SIX5
Human
IHC, Western blot, ELISA
FITC Conjugated
Anti-SIX5 Antibody (aa257-286, PE) LS-C240817
Rabbit Polyclonal (IgG) to Human SIX5
Human
IHC, Western blot, ELISA
PE Conjugated
Anti-SIX5 Antibody (aa257-286, Azide-free, HRP) LS-C248164
Rabbit Polyclonal (IgG) to Human SIX5
Human
IHC, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SIX5
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human SIX5
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

SIX5 antibody was raised against kLH-conjugated synthetic peptide between amino acids 257-286 from internal region of human SIX5.
LS-E1904 - Lyophilized - 100 µg - $145.00
aa257-286
Human SIX5

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SIX5

Q8N196 NP_787071.2

SIX5 Antibody, DMAHP Antibody, Homeobox protein SIX5 Antibody, Sine oculis homeobox homolog 5 Antibody, SIX homeobox 5 Antibody, BOR2 Antibody

Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter (By similarity). Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3.

Immunohistochemistry

Immunohistochemistry
SIX5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with SIX5 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.

Western blot

Western blot
SIX5 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The SIX5 antibody detected the SIX5 protein (arrow).
 

Requested From: United States
Date Requested: 12/7/2016

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