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SIX1 Antibody (C‑Terminus) LS‑C110311
SIX1 antibody LS-C110311 is an unconjugated rabbit polyclonal antibody to SIX1 from human and pig. Validated for WB.
Catalog
Size
Price
LS-C110311-50
50 µg
Unavailable

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Product Description

SIX1 antibody LS-C110311 is an unconjugated rabbit polyclonal antibody to SIX1 from human and pig. Validated for WB.
About SIX1
Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Q15475 NM_005982 NP_005973.1

SIX1 Antibody, BOS3 Antibody, DFNA23 Antibody, Sine oculis homeobox homolog 1 Antibody, SIX homeobox 1 Antibody, TIP39 Antibody, Homeobox protein SIX1 Antibody


Specifications

Target
Human SIX1
Host
Rabbit
Reactivity
Human, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
SIX1 antibody was raised against synthetic peptide from C-Terminus of human SIX1 (Q15475, NP_005973). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Panda, Dog, Bat, Rabbit, Guinea pig (92%); Marmoset, Horse, Pig, Xenopus (85%).
Blocking Peptide
LS-E13939 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110311. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human SIX1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIX1 antibody LS-C110311 Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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