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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIT1 Antibody (Intracellular, clone SIT‑01, PE) LS‑C46498
SIT1 antibody LS-C46498 is a PE-conjugated mouse monoclonal antibody to human SIT1. Validated for Flow, IP and WB.
Catalog
Size
Price
LS-C46498-100
100 µg (0.1 mg/ml)
$375

Popular SIT1 Products

Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, Flow Cytometry
Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01  and goat anti-mouse IgG (H+L)-HRP conjugate.
Species: Human
Applications: Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, Immunoprecipitation

Product Description

SIT1 antibody LS-C46498 is a PE-conjugated mouse monoclonal antibody to human SIT1. Validated for Flow, IP and WB.
About SIT1
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells. Q9Y3P8 NM_014450 NP_055265.1

SIT1 Antibody, gp30/40 Antibody, RP11-331F9.5 Antibody, SIT-R Antibody, SIT Antibody


Specifications

Target
Human SIT1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [SIT-01]
Conjugations
Phycoerythrin. Also available Unconjugated.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:50)
  • (applications tested for the base form of this product only)
Immunogen
SIT1 antibody was raised against bacterially produced recombinant intracellular fragment of human SIT.
Epitope
Intracellular
Specificity
The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.
Usage
The reagent is designed for Flow Cytometry analysis (see application note on <u>www.exbio.cz</u>). Suggested working dilution is 1:50. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 15 mM Sodium Azide
Storage
Store in the dark at 2°C to 8°C. Do not freeze. Protect from light.
Restrictions
For research use only.

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Images

Flow Cytometry

Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.
Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.

Flow Cytometry

Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.
Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.

Flow Cytometry

Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.
Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.

Flow Cytometry

Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.
Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.

Requested From: United States
Date Requested: 9/19/2018

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