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Anti-SIRT5 / Sirtuin 5 Antibody (clone 2E12) LS-C173179

Catalog	Size	Price
LS-C173179-100	100 µl (0.57 mg/ml)	Unavailable

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Anti-SIRT5 / Sirtuin 5 Antibody (clone 2A11) LS-C173171

Antibody:	Mouse Monoclonal [clone 2A11] (IgG1) to Human SIRT5 / Sirtuin 5
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Anti-SIRT5 / Sirtuin 5 Antibody (aa37-310) LS-C373325

Antibody:	Rabbit Polyclonal (IgG) to Human SIRT5 / Sirtuin 5
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Western blot of SIRT5 / Sirtuin 5 antibody.

Antibody:	Mouse Monoclonal [clone 2E12] (IgG1) to Human SIRT5 / Sirtuin 5
Reactivity:	Human
Application:	Western blot, Flow Cytometry
Format:	Unconjugated

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody, and then analyzed by flow cytometry.

Details

Target:	Human SIRT5 / Sirtuin 5
Host:	Mouse
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	IgG1 Monoclonal [2E12]
Conjugations:	Unconjugated
Purification:	Protein A/G purified
Modifications:	Unmodified

Applications

Approved:

Western blot (1:500)
Flow Cytometry (1:100)

Specificity and Use

Immunogen:	Full length human recombinant protein of human SIRT5 (NP_036373) produced in HEK293T cell.
Specificity:	Human SIRT5

Packaging

Presentation:	PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About SIRT5 / Sirtuin 5

Uniprot: Q9NXA8 NCBI: NM_031244 NP_112534.1

Synonyms: SIRT5 Antibody, SIR2L5 Antibody, Sir2-like 5 Antibody, SIR2-like protein 5 Antibody, Sirtuin 5 Antibody, Sirtuin type 5 Antibody

Description: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species.

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Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody, and then analyzed by flow cytometry.

Requested From:
Date Requested: 4/21/2018

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