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Anti-SIRT5 / Sirtuin 5 Antibody (clone 2E12) LS-C173179

Catalog	Size	Price
LS-C173179-100	100 µl (0.57 mg/ml)	Unavailable

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Antibody:	Mouse Monoclonal [clone 2E12] (IgG1) to Human SIRT5 / Sirtuin 5
Reactivity:	Human
Application:	Western blot, Flow Cytometry
Format:	Unconjugated

Details

Target:	Human SIRT5 / Sirtuin 5
Host:	Mouse
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	IgG1 Monoclonal [2E12]
Conjugations:	Unconjugated
Purification:	Protein A/G purified
Modifications:	Unmodified

Applications

Approved:

Western blot (1:500)
Flow Cytometry (1:100)

Specificity and Use

Immunogen:	Full length human recombinant protein of human SIRT5 (NP_036373) produced in HEK293T cell.
Specificity:	Human SIRT5

Packaging

Presentation:	PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About SIRT5 / Sirtuin 5

Uniprot: Q9NXA8 NCBI: NM_031244 NP_112534.1

Synonyms: SIRT5 Antibody, SIR2L5 Antibody, Sir2-like 5 Antibody, SIR2-like protein 5 Antibody, Sirtuin 5 Antibody, Sirtuin type 5 Antibody

Description: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species.

(More About SIRT5 / Sirtuin 5)

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Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody, and then analyzed by flow cytometry.

Requested From:
Date Requested: 8/19/2017

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