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Anti-SIRT4 / Sirtuin 4 Antibody (aa302-314) IHC-plus™ LS-B2493

Note: This antibody replaces LS-C55470

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SIRT4 / Sirtuin 4 Antibodies

Anti-SIRT4 / Sirtuin 4 Antibody (aa1-50) LS-C10183
Mouse Monoclonal (IgG1) to Human SIRT4 / Sirtuin 4
Human
ELISA
Unconjugated
Anti-SIRT4 / Sirtuin 4 Antibody (aa216-265) IHC-plus™ LS-B5590
Rabbit Polyclonal to Human SIRT4 / Sirtuin 4
Human, Monkey, Dog, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human SIRT4 / Sirtuin 4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SIRT4 / Sirtuin 4
Goat
Human (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Sheep (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA

Specificity and Use

SIRT4 / Sirtuin 4 antibody was raised against synthetic peptide SRCGELLPLIDPC from the C-terminus of human SIRT4 (NP_036372.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Bovine, Horse, Pig (92%); Dog, Platypus (85%).
LS-E25687 - Lyophilized - 100 µg - $145.00
aa302-314
Human SIRT4.
Immunohistochemistry: LS-B2493 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2493 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SIRT4 / Sirtuin 4

Q9Y6E7 NM_012240 NP_036372.1

SIRT4 Antibody, Sir2-like 4 Antibody, SIR2-like protein 4 Antibody, Sirtuin 4 Antibody, Sirtuin type 4 Antibody, SIR2L4 Antibody

Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity.

Immunohistochemistry

Immunohistochemistry
Anti-SIRT4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2493 concentration 5 ug/ml.

Western blot

Western blot
Antibody staining (1 ug/ml) of Human Kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: United States
Date Requested: 12/9/2016

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