LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
SIRT3 / Sirtuin 3 Antibody (aa170‑183) LS‑C139812
Sirtuin 3 antibody LS-C139812 is an unconjugated goat polyclonal antibody to Sirtuin 3 (SIRT3) from mouse, rat and pig. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C139812-100
100 µg (0.5 mg/ml)
Unavailable

Popular SIRT3 / Sirtuin 3 Products

Fetal Thymus Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: Western blot
Antibody
Species: Mouse
Applications: Western blot, ELISA
Western blot of various cell extracts using LS-C75838.
Species: Human, Rat
Applications: Western blot, Immunoprecipitation, ELISA
Anti-SIRT3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8047 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Sirtuin 3 antibody LS-C139812 is an unconjugated goat polyclonal antibody to Sirtuin 3 (SIRT3) from mouse, rat and pig. Validated for Peptide-ELISA and WB.
About SIRT3 / Sirtuin 3
SIRT3 / Sirtuin 3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Q9NTG7 NM_012239 NP_036371.1

SIRT3 Antibody, HSIRT3 Antibody, Sir2-like 3 Antibody, SIR2-like protein 3 Antibody, SIR2L3 Antibody, Sirtuin 3 Antibody, Sirtuin type 3 Antibody


Specifications

Target
Mouse SIRT3 / Sirtuin 3
Host
Goat
Reactivity
Mouse, Rat, Pig (tested or 100% immunogen sequence identity)
Predicted
Human, Monkey, Bovine, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Immunogen
Synthetic peptide C-RASGIPASKLVEAH from an internal region of mouse SIRT3 (NP_071878.2; NP_001171275.1). Percent identity by BLAST analysis: Mouse, Rat, Elephant, Pig (100%); Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bovine, Horse (93%); Dog, Rabbit (86%).
Blocking Peptide
LS-E27704 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139812. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa170-183
Specificity
Mouse SIRT3. This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 30kD band observed in Mouse Heart lysates (calculated MW of 28.8kD according to NP_071878.2). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 15kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/19/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number