Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 7B3] (IgG1) to Human SIRPA / CD172a
IHC - Paraffin, Western blot
Mouse Monoclonal (IgG2a) to Rat SIRPA / CD172a
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Rat SIRPA / CD172a
Rat (tested or 100% immunogen sequence identity)
Protein G purified
IHC - Paraffin
IHC - Frozen
Flow Cytometry (1:100 - 1:200)
Specificity and Use
SIRPA / CD172a antibody was raised against rat peritoneal macrophages.
Recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. Immunohistology: This product requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase.
PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
CD172A / SIRPA is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases.