Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone P84] (IgG1,k) to Mouse SIRPA / CD172a
Mouse SIRPA / CD172a
Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [P84]
Specificity and Use
SIRPA / CD172a antibody was raised against mouse SIRPA
This P84 antibody has been tested by flow cytometric analysis of mouse bone marrow. This can be used at less than or equal to 0.25 ug This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
CD172A / SIRPA is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases.
Staining of C57Bl/6 bone marrow cells with APC anti-mouse CD11b (M1/70) (LS-C107298) and 0.125 ug of Biotin anti-mouse SIRPa (CD172a). Cells in the lymphoid (left) and myeloid (right) gates were used for analysis.
Requested From: United States
Date Requested: 10/28/2016