Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone ED9] (IgG1) to Rat SIRPA / CD172a
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Rat SIRPA / CD172a
Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [ED9]
Protein A purified
IHC - Frozen (1:100 - 1:1000)
Flow Cytometry (1:10 - 1:50)
Specificity and Use
SIRPA / CD172a antibody was raised against spleen cell homogenate.
Recognizes rat CD172a also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. ED9 binds to a different epitope on SIRP than MRC OX-41 and has been reported to block the interaction of CD172a - CD47. Removal of Sodium Azide is recommended prior to use in functional assays. XXX recommend the use of EQU003 for this purpose.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
CD172A / SIRPA is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases.