Pig SIRPA / CD172a

Mouse

Pig (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

BL1H7

Unconjugated. Also available conjugated with FITC.

Protein G purified

Unmodified

Porcine alveolar macrophages.

Porcine CD172a, a member of the signal regulatory protein (SIRP) family. Clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (;). CD172a is expressed on monocyte derived dendritic cells (MoDCs) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(; ). Clone BL1H7 immunoprecipitated a single band of ~90-110kD from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions. Has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues. Aberrant expression of CD172a has been noted on porcine leukemias with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a as opposed to the more characteristic of CD172+ cells seen in normal blood PBMC.

• IHC - Paraffin
• IHC - Frozen
• Western blot
• Immunoprecipitation
• Flow Cytometry

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P78324 NCBI: NM_080792 NP_542970.1