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Anti-SIN3A Antibody (N-Terminus) IHC-plus™ LS-B3492

Note: This antibody replaces LS-C101395

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SIN3A Antibodies

Anti-SIN3A Antibody (N-Terminus) IHC-plus™ LS-B3492
Rabbit Polyclonal (IgG) to Human SIN3A
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SIN3A Antibody IHC-plus™ LS-B10248
Rabbit Polyclonal (IgG) to Mouse SIN3A
Mouse, Human, Rat, Hamster
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human SIN3A
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SIN3A
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Guinea pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

SIN3A antibody was raised against synthetic peptide from N-Terminus of human SIN3A (Q96ST3, NP_056292). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig (100%); Rabbit, Opossum, Guinea pig (92%); Zebra finch, Platypus (84%).
LS-E11389 - Lyophilized - 100 µg - $145.00
N-Terminus
Human SIN3A
Immunohistochemistry: LS-B3492 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3492 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SIN3A

Q96ST3 NM_015477 NP_056292.1

SIN3A Antibody, DKFZP434K2235 Antibody, KIAA0700 Antibody

Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in he control of the circadian rhythms.

Immunohistochemistry

Immunohistochemistry
Anti-SIN3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3492 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-SIN3A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3492 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
SIN3A antibody LS-B3492 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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