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Anti-SIM2 Antibody (N-Terminus) LS-C110289

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Related Products

8 SIM2 Antibodies

Most Popular SIM2 Antibodies

Anti-SIM2 Antibody (N-Terminus) LS-C110289
Rabbit Polyclonal to Human SIM2
Human, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Western blot Image
Anti-SIM2 Antibody (clone 3E6) LS-C198198
Mouse Monoclonal [clone 3E6] (IgG2a,k) to Human SIM2
Human
Western blot, ELISA
Unconjugated
Enzyme-Linked Immunosorbent Assay Image
Anti-SIM2 Antibody (N-Terminus, Biotin) LS-C437165
Rabbit Polyclonal to Human SIM2
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Biotin Conjugated
Western blot Image
Anti-SIM2 Antibody (N-Terminus, FITC) LS-C437166
Rabbit Polyclonal to Human SIM2
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
FITC Conjugated
Western blot Image
Anti-SIM2 Antibody (N-Terminus, HRP) LS-C437167
Rabbit Polyclonal to Human SIM2
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
HRP Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SIM2
Human, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SIM2
Rabbit
Human, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Bat, Bovine, Hamster, Pig, Drosophila (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

SIM2 antibody was raised against synthetic peptide from N-Terminus of human SIM2 (Q14190, NP_005060). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Horse, Opossum, Guinea pig, Zebra finch, Chicken, Lizard, Xenopus, Zebrafish (100%); Orangutan, Tamarin, Hamster, Panda, Bovine, Bat, Pig, Platypus, Stickleback, Drosophila (92%); Beetle (85%).
LS-E13868 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110289. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Human SIM2
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SIM2

Q14190 NM_005069 NP_005060.1

SIM2 Antibody, BHLHe15 Antibody, HMC29C01 Antibody, HMC13F06 Antibody, Transcription factor SIM2 Antibody, Single-minded homolog 2 Antibody, SIM Antibody

This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes.

Western blot

Western blot
SIM2 antibody LS-C110289 Western blot of Fetal Kidney lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/27/2017

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