Target
Human SIM2
Synonyms
SIM2 | BHLHe15 | HMC29C01 | HMC13F06 | Transcription factor SIM2 | Single-minded homolog 2 | SIM
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-SDLLYTPSYS, from the internal region of the protein sequence according to NP_005060.1NP_033664.2.
Specificity
Human SIM2. This antibody is expected to recognize all reported isoforms (NP_005060.1; NP_033664.2).
Applications
- Western blot (0.5 - 2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Usage
Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 80kD band observed in fetal Mouse Kidney lysates (calculated MW of 73.2kD according to Human NP_005060.1) and 72.5kD according to Mouse NP_035507.2. Recommended concentration: 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee