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| Catalog Number | Size | Price |
|---|---|---|
| LS-C45080-0.1 | 0.1 mg (0.5 mg/ml) | $395 |
Monoclonal Mouse anti‑Rat SIGLEC1 / CD169 / Sialoadhesin Antibody (clone ED3, IHC, IF) LS‑C45080
Monoclonal Mouse anti‑Rat SIGLEC1 / CD169 / Sialoadhesin Antibody (clone ED3, IHC, IF) LS‑C45080
Note: This antibody replaces LS-C45081
Antibody:
SIGLEC1 / CD169 / Sialoadhesin Mouse anti-Rat Monoclonal (ED3) Antibody
Application:
IHC, IHC-Fr, IF, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
SIGLEC1 / CD169 / Sialoadhesin Mouse anti-Rat Monoclonal (ED3) Antibody
Application:
IHC, IHC-Fr, IF, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Specifications
Description
Sialoadhesin antibody LS-C45080 is an unconjugated mouse monoclonal antibody to rat Sialoadhesin (SIGLEC1 / CD169). Validated for Flow, IF, IHC and IP.
Target
Rat SIGLEC1 / CD169 / Sialoadhesin
Synonyms
SIGLEC1 | CD169 | CD169 antigen | SIGLEC-1 | Sialoadhesin | SN | DJ1009E24.1
Host
Mouse
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
ED3
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Rat spleen cell homogenate.
Specificity
Recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicuous property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
Applications
- IHC
- IHC - Frozen (1:250)
- Immunofluorescence
- Immunoprecipitation
- Flow Cytometry (1:500)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SIGLEC1 / CD169 / Sialoadhesin
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Flow Cytometry, ELISA, Radioimmunoassay
Species:
Pig
Applications:
IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Mouse
Applications:
Immunofluorescence, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Species:
Mouse
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
