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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIGLEC1 / CD169 / Sialoadhesin Antibody LS‑C45080
Note: This antibody replaces LS-C45081
Sialoadhesin antibody LS-C45080 is an unconjugated mouse monoclonal antibody to rat Sialoadhesin (SIGLEC1 / CD169). Validated for Flow, IHC and IP.
Catalog
Size
Price
LS-C45080-100
100 µg (0.5 mg/ml)
$365
Description
Sialoadhesin antibody LS-C45080 is an unconjugated mouse monoclonal antibody to rat Sialoadhesin (SIGLEC1 / CD169). Validated for Flow, IHC and IP.
Target
Rat SIGLEC1 / CD169 / Sialoadhesin
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen (1:250)
  • Immunoprecipitation
  • Flow Cytometry (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Rat spleen cell homogenate.
Specificity
Recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicuous property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Restrictions
For research use only.
About SIGLEC1 / CD169 / Sialoadhesin
Q9BZZ2 NM_023068 NP_075556.1

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Requested From: United States
Date Requested: 12/9/2018
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