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Anti-SIAH2 Antibody (aa215-264) LS-C109768

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Most Popular SIAH2 Antibodies

Anti-SIAH2 Antibody (N-Terminus) LS-C112149
Rabbit Polyclonal to Human SIAH2
Human, Monkey, Mouse
Western blot
Unconjugated
Western blot Image
Anti-SIAH2 Antibody (N-Terminus, clone 24E6H3) LS-C141889
Mouse Monoclonal [clone 24E6H3] (IgG1) to Drosophila SIAH2
Drosophila, Human, Pig
IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-SIAH2 Antibody (aa216-244) LS-C161085
Rabbit Polyclonal to Human SIAH2
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SIAH2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SIAH2
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Hamster, Drosophila (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

  • IHC - Paraffin

Specificity and Use

SIAH2 antibody was raised against synthetic peptide located between aa215-264 of human SIAH2 (O43255, NP_005058). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Xenopus, Zebrafish (100%); Hamster, Panda, Zebra finch, Stickleback, Drosophila (92%).
LS-E11735 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109768. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa215-264
Human SIAH2
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SIAH2

O43255 NM_005067 NP_005058.3

SIAH2 Antibody, HSiah2 Antibody, Seven in absentia homolog 2 Antibody, Siah-2 Antibody

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes.

Western blot

Western blot
SIAH2 antibody LS-C109768 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/26/2017

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