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Anti-SHPRH Antibody (aa251-300) LS-C110174

Catalog Size Price
LS-C110174-50 50 µg Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human SHPRH
Human, Mouse, Rat, Bat, Hamster, Horse, Pig, Rabbit
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SHPRH
Rabbit
Human, Mouse, Rat, Bat, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Monkey, Bovine, Dog, Guinea pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

SHPRH antibody was raised against synthetic peptide located between aa251-300 of human SHPRH (Q149N8-4, NP_775105). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Rabbit, Horse, Pig (100%); Monkey, Galago, Dog, Bovine, Guinea pig (92%).
LS-E13087 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110174. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa251-300
Human SHPRH
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SHPRH

Q149N8 AA476814 NP_775105.1

SHPRH Antibody, BA545I5.2 Antibody, KIAA2023 Antibody

E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.

Western blot

Western blot
SHPRH antibody LS-C110174 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 6/23/2017

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