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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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SHOX Antibody (N‑Terminus) LS‑C101436
SHOX antibody LS-C101436 is an unconjugated rabbit polyclonal antibody to SHOX from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C101436-50
50 µg
Unavailable

Popular SHOX Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
All lanes: Anti-SHOX Antibody (N-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: human pancreas lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

SHOX antibody LS-C101436 is an unconjugated rabbit polyclonal antibody to SHOX from human, mouse, rat and other species. Validated for WB.
About SHOX
The pseudoautosomal region (PAR1) contains an interval of 170 kb that tends to be deleted in individuals with short stature and different rearrangements on Xp22 or Yp11.3. SHOX (Short stature HOmeoboX-containing gene) a homeo box-containing gene located in the PAR1, has at least 2 alternatively spliced forms encoding proteins with different patterns of expression. SHOX is involved in idiopathic growth retardation and in the short stature phenotype of Turner syndrome patients. O15266 NM_000451 NP_000442.1

SHOX Antibody, GCFX Antibody, PHOG Antibody, Short stature homeobox Antibody, Short stature homeobox protein Antibody, SS Antibody, SHOXY Antibody


Specifications

Target
Human SHOX
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig, Chicken (tested or 100% immunogen sequence identity)
Predicted
Bovine, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
SHOX antibody was raised against synthetic peptide from N-Terminus of human SHOX (O15266, NP_000442). Percent identity by BLAST analysis: Human, Gorilla, Galago, Elephant, Panda, Dog, Pig, Chicken (100%); Bovine (92%); Stickleback, Zebrafish (90%); Monkey, Marmoset, Mouse, Rat, Guinea pig (81%).
Blocking Peptide
LS-E11731 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101436. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human SHOX
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SHOX antibody LS-C101436 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SHOX antibody LS-C101436 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SHOX antibody LS-C101436 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SHOX antibody LS-C101436 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SHOX antibody LS-C101436 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SHOX antibody LS-C101436 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SHOX antibody LS-C101436 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SHOX antibody LS-C101436 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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