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Anti-SHMT / SHMT1 Antibody (clone 4F9) IHC-plus™ LS-B4417

Note: This antibody replaces LS-C105027

Ordering

Wt. Vol. Conc. Price
50 µg - 0.46 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SHMT / SHMT1 Antibodies

Anti-SHMT / SHMT1 Antibody (clone 4F9) IHC-plus™ LS-B4417
Mouse Monoclonal [clone 4F9] (IgG1,k) to Human SHMT / SHMT1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SHMT / SHMT1 Antibody (aa453-483) IHC-plus™ LS-B12658
Rabbit Polyclonal to Human SHMT / SHMT1
Human, Mouse, Dog, Guinea pig
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4F9] (IgG1,k) to Human SHMT / SHMT1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SHMT / SHMT1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [4F9]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

SHMT / SHMT1 antibody was raised against sHMT1 (NP_004160, 374 a.a. ~ 483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control HeLa. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein).

Packaging

PBS, pH 7.4. Sourced in Ascites.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About SHMT / SHMT1

P34896 NM_004169 NP_004160.3

SHMT1 Antibody, 14 kDa protein Antibody, CSHMT Antibody, Serine methylase Antibody, SHMT Antibody

SHMT / SHMT1 is the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1.

Immunohistochemistry

Immunohistochemistry
Anti-SHMT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4417 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-SHMT1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4417 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-SHMT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4417 concentration 5 ug/ml.

Western blot

Western blot
Western blot of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody clone 4F9.

Western blot

Western blot
SHMT1 monoclonal antibody clone 4F9 Western blot of SHMT1 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged SHMT1 is approximately 1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/7/2016

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