LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
SHH / Sonic Hedgehog Antibody (clone 3A2) LS‑C114335
Sonic Hedgehog antibody LS-C114335 is an unconjugated mouse monoclonal antibody to human Sonic Hedgehog (SHH). Validated for IF and WB.
Catalog
Size
Price
LS-C114335-100
100 µl (0.12 mg/ml)
Unavailable

Popular SHH / Sonic Hedgehog Products

SHH antibody LS-C40460 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-Sonic Hedgehog / SHH antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Sonic Hedgehog antibody IHC-paraffin: Human Mammary Tissue.
Species: Human, Orangutan, Monkey, Mouse, Rat, Bovine, Dog, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Sonic Hedgehog antibody LS-C114335 is an unconjugated mouse monoclonal antibody to human Sonic Hedgehog (SHH). Validated for IF and WB.
About SHH / Sonic Hedgehog
SHH / Sonic Hedgehog is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. Q15465 NM_000193 NP_000184.1

SHH Antibody, HHG-1 Antibody, HLP3 Antibody, Sonic hedgehog Antibody, Sonic hedgehog homolog Antibody, MCOPCB5 Antibody, Sonic hedgehog protein Antibody, HHG1 Antibody, HPE3 Antibody, SMMCI Antibody, TPT Antibody, TPTPS Antibody


Specifications

Target
Human SHH / Sonic Hedgehog
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [3A2]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
Immunogen
Recombinant protein expressed in E. coli corresponding to amino acids 24-197 of human SHH.
Specificity
Human Sonic Hedgehog / SHH
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunofluorescence

Anti-SHH mouse monoclonal antibody  immunofluorescent staining  (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
Anti-SHH mouse monoclonal antibody immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.

Immunofluorescence

Anti-SHH mouse monoclonal antibody  immunofluorescent staining  (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
Anti-SHH mouse monoclonal antibody immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.

Immunofluorescence

Anti-SHH mouse monoclonal antibody  immunofluorescent staining  (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
Anti-SHH mouse monoclonal antibody immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.

Immunofluorescence

Anti-SHH mouse monoclonal antibody  immunofluorescent staining  (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.
Anti-SHH mouse monoclonal antibody immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.


Requested From: 
Date Requested: 6/23/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number