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Anti-SHH / Sonic Hedgehog Antibody (clone 3A2) LS-C114335

Catalog Size Price
LS-C114335-100 100 µl (0.12 mg/ml) Unavailable

Most Popular SHH / Sonic Hedgehog Antibodies

Anti-SHH / Sonic Hedgehog Antibody (N-Terminus) LS-C40460
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Human, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SHH / Sonic Hedgehog Antibody (C-Terminus) LS-C150253
Goat Polyclonal (IgG) to Mouse SHH / Sonic Hedgehog
Mouse, Human
IHC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SHH / Sonic Hedgehog Antibody (clone 5H4) LS-C169089
Mouse Monoclonal [clone 5H4] (IgG1) to Human SHH / Sonic Hedgehog
Human, Monkey, Mouse
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SHH / Sonic Hedgehog Antibody (clone 8G3) IHC-plus™ LS-B8934
Mouse Monoclonal [clone 8G3] (IgG1) to Human SHH / Sonic Hedgehog
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SHH / Sonic Hedgehog Antibody (aa36-193, Biotin) LS-C421148
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Human
ICC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed
Mouse Monoclonal [clone 3A2] (IgG2a) to Human SHH / Sonic Hedgehog
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human SHH / Sonic Hedgehog
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [3A2]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:2000)

Specificity and Use

SHH / Sonic Hedgehog antibody was raised against recombinant protein expressed in E. coli corresponding to amino acids 24-197 of human SHH.
Human Sonic Hedgehog / SHH
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SHH / Sonic Hedgehog

Q15465 NM_000193 NP_000184.1

SHH Antibody, HHG-1 Antibody, HLP3 Antibody, Sonic hedgehog Antibody, Sonic hedgehog homolog Antibody, MCOPCB5 Antibody, Sonic hedgehog protein Antibody, HHG1 Antibody, HPE3 Antibody, SMMCI Antibody, TPT Antibody, TPTPS Antibody

SHH / Sonic Hedgehog is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar.

Immunofluorescence

Immunofluorescence
Anti-SHH mouse monoclonal antibody immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.

Requested From: 
Date Requested: 6/22/2017

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