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Anti-SHH / Sonic Hedgehog Antibody (aa199-437) LS-C10144

Ordering

Wt. Vol. Conc. Price
100 µg - - $325
500 µg - - $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SHH / Sonic Hedgehog Antibodies

Anti-SHH / Sonic Hedgehog Antibody (N-Terminus) LS-C40460
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Human, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SHH / Sonic Hedgehog Antibody (clone 8G3) IHC-plus™ LS-B8934
Mouse Monoclonal [clone 8G3] (IgG1) to Human SHH / Sonic Hedgehog
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG2a) to Mouse SHH / Sonic Hedgehog
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse SHH / Sonic Hedgehog
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (1 - 2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

SHH / Sonic Hedgehog antibody was raised against purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide; aa 199-437).
aa199-437
Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not cross-react with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide.
Suitable for use in Western Blot and ELISA. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for rmShh C-terminal peptide is approximately 1 ng/well. Western Blot: 1-2 ug/ml with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for recombinant mouse Shh C-terminal peptide is approximately 0.5 ng/lane under non-reducing and reducing conditions.

Packaging

PBS, pH 7.2, 5% trehalose.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About SHH / Sonic Hedgehog

Q15465 NM_000193 NP_000184.1

SHH Antibody, HHG-1 Antibody, HLP3 Antibody, Sonic hedgehog Antibody, Sonic hedgehog homolog Antibody, MCOPCB5 Antibody, Sonic hedgehog protein Antibody, HHG1 Antibody, HPE3 Antibody, SMMCI Antibody, TPT Antibody, TPTPS Antibody

SHH / Sonic Hedgehog is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar.

Requested From: United States
Date Requested: 12/10/2016

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