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Anti-SGSH Antibody (aa318-496) LS-C185423

Catalog Size Price
LS-C185423-100 100 µl (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human SGSH
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human SGSH
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:1000 - 1:10000)

Specificity and Use

SGSH antibody was raised against recombinant fragment corresponding to a region within amino acids 318 and 496 of SGSH (SwissProt P51688). Percent identity by BLAST analysis: Human (100%); Dog (82%); Mouse, Bovine (81%).
aa318-496
Human SGSH
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About SGSH

P51688 NM_000199 NP_000190.1

SGSH Antibody, HSS Antibody, Heparan sulfate sulfatase Antibody, MPS3A Antibody, Sulphamidase Antibody, Sulfoglucosamine sulfamidase Antibody, SFMD Antibody

SGSH encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded OVCAR3 xenograft using SGSH antibody at 1:100 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. SGSH antibody diluted at 1:5000.

Requested From: 
Date Requested: 10/19/2017

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