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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SGK1 / SGK Antibody (phospho‑Ser422) LS‑C199563
SGK antibody LS-C199563 is an unconjugated rabbit polyclonal antibody to SGK (SGK1) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199563-50
50 µl
Unavailable
LS-C199563-100
100 µl
Unavailable

Popular SGK1 / SGK Products

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Anti-SGK1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5532 concentration 5 ug/ml.
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Antibody
Species: Mouse, Human
Applications: Western blot, ELISA

Product Description

SGK antibody LS-C199563 is an unconjugated rabbit polyclonal antibody to SGK (SGK1) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About SGK1 / SGK
Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. O00141 NM_005627 NP_005618.2

SGK1 Antibody, Hsgk Antibody, Hsgk1 Antibody, SGK Antibody, Sgk1 variant i3 Antibody


Specifications

Target
Human SGK1 / SGK
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SGK1 / SGK antibody was raised against synthetic peptide derived from human SGK around the phosphorylation site of Ser422.
Epitope
pSer422
Specificity
SGK (Phospho-Ser422) Antibody detects endogenous levels of SGK only when phosphorylated at serine422.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/24/2018

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