Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (5 µg/ml)
Western blot (0.01 - 0.03 µg/ml)
Specificity and Use
SFTPA1 + SFTPA2 antibody was raised against synthetic peptide C-HLDEELQATLHDFR from an internal region of human SFTPA1 / Surfactant Protein A (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1; NP_001092138.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%); Marmoset (86%).
Human SFTPA1 / Surfactant Protein A. This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 30kD band observed in Human Lung lysates (calculated MW of 27.7kD according to NP_001087239.2). Recommended concentration: 0.01-0.03 ug/ml. Immunohistochemistry: 5 ug/ml.
Anti-SFTPA1 / Surfactant Protein A antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9818 concentration 5 ug/ml.
SFTPA1 antibody (0.01 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.