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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SFTPA1 / Surfactant Protein A Antibody IHC-plus™ LS-B2543

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Note: This antibody replaces LS-C17957
Surfactant Protein A antibody LS-B2543 is an unconjugated mouse monoclonal antibody to human Surfactant Protein A (SFTPA1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B2543-50
50 µl (1 mg/ml)
$425
Description
Surfactant Protein A antibody LS-B2543 is an unconjugated mouse monoclonal antibody to human Surfactant Protein A (SFTPA1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human SFTPA1 / Surfactant Protein A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500)
  • ELISA (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Native SP-A purified from bronchioalveolar lavage from proteinosis patients.
Specificity
Recognizes human pulmonary SP-A. In Western Blot after SDS-PAGE it reacts with reduced forms of SP-A and weaker with non-reduced forms. In immunohistochemical staining from paraffin embedded tissue samples, this antibody reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. Reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.
Usage
Immunohistochemistry: LS-B2543 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2543 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SFTPA1 / Surfactant Protein A
Q8IWL2 NM_005411 NP_005402.3

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Publications (1)

1
High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins. Jin H, Zangar RC. Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]. 2012 Unit 17.15. [PubMed:22511115]

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Images

Immunohistochemistry

Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Requested From: United States
Date Requested: 4/23/2019
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