LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-SFTPA1 / Surfactant Protein A Antibody IHC-plus™ LS-B2543

Note: This antibody replaces LS-C17957

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SFTPA1 / Surfactant Protein A Antibodies

Anti-SFTPA1 / Surfactant Protein A Antibody IHC-plus™ LS-B2543
Mouse Monoclonal (IgG1,k) to Human SFTPA1 / Surfactant Protein A
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SFTPA1 / Surfactant Protein A Antibody (aa233-248) LS-C312616
Rabbit Polyclonal (IgG) to Mouse SFTPA1 / Surfactant Protein A
Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal (IgG1,k) to Human SFTPA1 / Surfactant Protein A
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SFTPA1 / Surfactant Protein A
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500)
  • ELISA (1:8000)

Specificity and Use

SFTPA1 / Surfactant Protein A antibody was raised against native SP-A purified from bronchioalveolar lavage from proteinosis patients.
Recognizes human pulmonary SP-A. In Western Blot after SDS-PAGE it reacts with reduced forms of SP-A and weaker with non-reduced forms. In immunohistochemical staining from paraffin embedded tissue samples, this antibody reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. Reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.
Immunohistochemistry: LS-B2543 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2543 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4, 15mM sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins. Jin H, Zangar RC. Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]. 2012 Unit 17.15. [PubMed:22511115]

About SFTPA1 / Surfactant Protein A

Q8IWL2 NM_005411 NP_005402.3

SFTPA1 Antibody, Alveolar proteinosis protein Antibody, COLEC4 Antibody, Collectin-4 Antibody, PSP-A Antibody, PSPA Antibody, SFTP1 Antibody, SFTPA Antibody, SFTPA1B Antibody, SPA1 Antibody, SP-A Antibody, SP-A1 Antibody, SPA Antibody, Surfactant Protein A Antibody, Surfactant protein A1 Antibody, Surfactant protein A1B Antibody

SFTPA1 / Surfactant Protein A is a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants.

Immunohistochemistry

Immunohistochemistry
Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2543 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number