Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
SFRP5 Antibody, FRP-1b Antibody, Frizzled-related protein 5 Antibody, SFRP-5 Antibody, SARP-3 Antibody, Frizzled-related protein 1b Antibody, FRP1B Antibody, SARP3 Antibody
SARP3 is a frizzled protein that lacks the 7-transmembrane motif used as anchor to the plasma membrane. They modulate Wnt signaling by competing with membrane-bound frizzled receptors for binding secreted Wnt ligands. SARP3 is involved in determining tissue of photoreceptor cells in the retina. It is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with SFRP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of SFRP5 Antibody in K562 cell line lysates (35 ug/lane). SFRP5 (arrow) was detected using the purified antibody.
Flow cytometric of K562 cells using SFRP5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.