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Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (N-Terminus) IHC-plus™ LS-B3281

Note: This antibody replaces LS-C42375

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SFN / Stratifin / 14-3-3 Sigma Antibodies

Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (clone 1433S01, Azide-free) LS-C87600
Mouse Monoclonal [clone 1433S01] (IgG1,k) to Human SFN / Stratifin / 14-3-3 Sigma
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (aa204-217) IHC-plus™ LS-B6688
Goat Polyclonal to Human SFN / Stratifin / 14-3-3 Sigma
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (aa222-248) LS-C169011
Rabbit Polyclonal to Human SFN / Stratifin / 14-3-3 Sigma
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Sheep SFN / Stratifin / 14-3-3 Sigma
Sheep, Human, Mouse, Rat, Bovine, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated

Details

Sheep SFN / Stratifin / 14-3-3 Sigma
Rabbit
Sheep, Human, Mouse, Rat, Bovine, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Mammal (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Unmodified

Applications

  • IHC - Paraffin (1:250)
  • Western blot

Specificity and Use

SFN / Stratifin / 14-3-3 Sigma antibody was raised against synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 sigma.
N-Terminus
Recognizes 14-3-3 sigma in all mammals. It is a member of the 14-3-3 family which consists of 30 kD proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1. May not react with recombinant proteins that are not N-acetylated.
Immunohistochemistry: LS-B3281 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3281 was determined to be 1:250. Western Blot: LS-B3281 detects a band of approximately 30kD in HEK293 cell lysates.

Packaging

Serum, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SFN / Stratifin / 14-3-3 Sigma

P31947 NM_006142 NP_006133.1

SFN Antibody, 14-3-3 sigma Antibody, 14-3-3 protein sigma Antibody, HME1 Antibody, Stratifin Antibody, YWHAS Antibody

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.

Immunohistochemistry

Immunohistochemistry
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3281 dilution 1:250.
 

Requested From: United States
Date Requested: 12/3/2016

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