Target
Human SFN / Stratifin / 14-3-3 Sigma
Synonyms
SFN | 14-3-3 sigma | 14-3-3 protein sigma | HME1 | Stratifin | YWHAS
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
GST fusion protein corresponding to full-length human 14-3-3 sigma.
Specificity
Recognizes human 14-3-3 sigma, Mr 30kD. Does not cross-react with other 14-3-3 isoforms. Species cross-reactivity: rat and mouse.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
WB: EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with Mouse Anti-14-3-3, sigma. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.014 M PB, pH 7.6, 0.175 M NaCl, 0.07% Sodium Azide, 30% Glycerol
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFN / Stratifin / 14-3-3 Sigma