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Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (aa179-228) IHC-plus™ LS-B5855

Note: This antibody replaces LS-C102566
Catalog Size Price
LS-B5855-50 50 µg (1 mg/ml) Unavailable

Most Popular SFN / Stratifin / 14-3-3 Sigma Antibodies

Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (clone 1433S01, Azide-free) LS-C87600
Mouse Monoclonal [clone 1433S01] (IgG1,k) to Human SFN / Stratifin / 14-3-3 Sigma
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated, Azide-free
Formalin-fixed, paraffin-embedded human skin stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (N-Terminus) IHC-plus™ LS-B3281
Rabbit Polyclonal (IgG) to Sheep SFN / Stratifin / 14-3-3 Sigma
Sheep, Human, Mouse, Rat, Bovine, Rabbit, Chicken
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3281 dilution 1:250.
Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (aa179-228) IHC-plus™ LS-B5855
Rabbit Polyclonal (IgG) to Human SFN / Stratifin / 14-3-3 Sigma
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5855 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (aa204-217) IHC-plus™ LS-B6688
Goat Polyclonal to Human SFN / Stratifin / 14-3-3 Sigma
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Sheep
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6688 concentration 2.5 ug/ml.
Anti-SFN / Stratifin / 14-3-3 Sigma Antibody (aa222-248) LS-C169011
Rabbit Polyclonal to Human SFN / Stratifin / 14-3-3 Sigma
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
SFN antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human SFN / Stratifin / 14-3-3 Sigma
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep
IHC, IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SFN / Stratifin / 14-3-3 Sigma
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Hamster (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

Synthetic peptide located between aa179-228 of human SFN (P31947, NP_006133). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch (100%); Hamster, Platypus (92%); Trout, Salmon, Zebrafish, Slime mold (85%).
LS-E19750 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5855. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa179-228
Human SFN / 14-3-3 Sigma
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Packaging

Lyophilized from PBS with 2% sucrose
Reconstitute with 50ul sterile ddH2O.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About SFN / Stratifin / 14-3-3 Sigma

P31947 NM_006142 NP_006133.1

SFN Antibody, 14-3-3 sigma Antibody, 14-3-3 protein sigma Antibody, HME1 Antibody, Stratifin Antibody, YWHAS Antibody

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5855 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5855 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SFN / 14-3-3 Sigma antibody LS-B5855 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SFN / 14-3-3 Sigma antibody LS-B5855 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/21/2018

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