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Anti-SF3B130 / SF3B3 Antibody (aa1043-1092) LS-C116181

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Most Popular SF3B130 / SF3B3 Antibodies

Anti-SF3B130 / SF3B3 Antibody (aa386-399) LS-C55433
Goat Polyclonal to Human SF3B130 / SF3B3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Western blot, ELISA
Unconjugated
Western blot Image
Anti-SF3B130 / SF3B3 Antibody (C-Terminus) IHC-plus™ LS-B12599
Rabbit Polyclonal (IgG) to Human SF3B130 / SF3B3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image
Anti-SF3B130 / SF3B3 Antibody (aa35-210) LS-C425167
Rabbit Polyclonal (IgG) to Mouse SF3B130 / SF3B3
Mouse
IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
Unconjugated
Anti-SF3B130 / SF3B3 Antibody (aa1167-1217) IHC-plus™ LS-B14434
Rabbit Polyclonal (IgG) to Human SF3B130 / SF3B3
Human, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image
Anti-SF3B130 / SF3B3 Antibody (aa1167-1217) IHC-plus™ LS-B14465
Rabbit Polyclonal (IgG) to Human SF3B130 / SF3B3
Human, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Immunofluorescence
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SF3B130 / SF3B3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SF3B130 / SF3B3
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

SF3B130 / SF3B3 antibody was raised against synthetic peptide located between aa1043-1092 of human SF3B3 (Q15393, NP_036558). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus (100%); Xenopus (92%); Salmon, Stickleback, Zebrafish (85%).
LS-E14919 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116181. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa1043-1092
Human SF3B3
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SF3B130 / SF3B3

Q15393 NM_012426 NP_036558.3

SF3B3 Antibody, RSE1 Antibody, Splicing factor 3B subunit 3 Antibody, SAP 130 Antibody, STAF130 Antibody, KIAA0017 Antibody, SF3b130 Antibody

SF3B130 / SF3B3 is subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome.

Western blot

Western blot
SF3B3 antibody LS-C116181 Western blot of HCT15 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/27/2017

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