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Anti-SF1 Antibody (aa10-25) IHC-plus™ LS-B3095

Note: This antibody replaces LS-C10333

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Most Popular SF1 Antibodies

Anti-SF1 Antibody (N-Terminus) LS-C31720
Rabbit Polyclonal (IgG) to Human SF1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SF1 Antibody (aa517-566) IHC-plus™ LS-B1563
Rabbit Polyclonal (IgG) to Human SF1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SF1 Antibody (aa10-25) IHC-plus™ LS-B3095
Rabbit Polyclonal (IgG) to Human SF1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human SF1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit
IHC - Paraffin, ELISA
Unconjugated

Details

Human SF1
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity)
Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ELISA

Specificity and Use

SF1 antibody was raised against synthetic peptide corresponding to aa10-25, LDFPSKKRKRSRWNQD of human SF1 (KLH). MW: 68.3kD. Percent identity by BLAST analysis: Human, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Bovine, Bat, Dog, Rabbit, Horse (100%); Gorilla, Panda, Pig, Armadillo (94%).
aa10-25
Recognizes human SF1. Species cross-reactivity: Rodent.
Immunohistochemistry: LS-B3095 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3095 was determined to be 10 ug/ml. Western Blot: Preliminary results: K562 cell line 50, 60, 100kD, HeLa 60, 65, 70, 100, 110kD. Human lung 65, 75kD.

Packaging

PBS, 0.02% sodium azide, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SF1

Q15637 NM_004630 NP_004621.2

SF1 Antibody, D11S636 Antibody, MBBP Antibody, ZCCHC25 Antibody, Transcription factor ZFM1 Antibody, Zinc finger gene in MEN1 locus Antibody, Zinc finger protein 162 Antibody, BBP Antibody, ZNF162 Antibody, Splicing factor 1 Antibody, ZFM1 Antibody

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Immunohistochemistry

Immunohistochemistry
Anti-SF1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3095 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-SF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3095 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-SF1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3095 concentration 10 ug/ml.
 

Requested From: 
Date Requested: 3/23/2017

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