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Anti-SETD8 / SET8 Antibody (N-Terminus, clone 43AT551.86) IHC-plus™ LS-B5237

Note: This antibody replaces LS-C96454

Ordering

Wt. Vol. Conc. Price
- 200 µl 0.25 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SETD8 / SET8 Antibodies

Anti-SETD8 / SET8 Antibody (N-Terminus, clone 43AT551.86) IHC-plus™ LS-B5237
Mouse Monoclonal [clone 43AT551.86] (IgG1) to Human SETD8 / SET8
Human, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SETD8 / SET8 Antibody IHC-plus™ LS-B14225
Rabbit Polyclonal (IgG) to Human SETD8 / SET8
Human, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 43AT551.86] (IgG1) to Human SETD8 / SET8
Human, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human SETD8 / SET8
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [43AT551.86]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • ICC (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:100 - 1:500)

Specificity and Use

SETD8 / SET8 antibody was raised against recombinant GST fusion protein encoding the N-terminal region of human SET07.
N-Terminus
Please note that cross-reactivity of this antibody with mouse has been verified in WB only. The antibody has not been evaluated for use in alternate assays with mouse samples. The antibody is not recommended for use in IHC-P with mouse samples due to the potential for issues with high background.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SETD8 / SET8

Q9NQR1 NM_020382 NP_065115.3

SETD8 Antibody, KMT5A Antibody, Lysine N-methyltransferase 5A Antibody, PR/SET07 Antibody, PRSET7 Antibody, SET8 Antibody, H4-K20-HMTase SETD8 Antibody, PR-Set7 Antibody, SET07 Antibody

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes.

Immunohistochemistry

Immunohistochemistry
Anti-SETD8 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5237 dilution 1:50.

Immunofluorescence

Immunofluorescence
(TOP)Negative control of HeLa cells without PE-conjugated goat anti-mouse lgG (whole molecule). PE-conjugated goat anti-mouse lgG emits red fluorescence. ( Bottom)Immunofluorescence of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).

Western blot

Western blot
Western blot of lysates from 293, 293T cell line (from left to right), using SET07 Antibody(43AT551. 86. 76). 43AT551. 86. 76 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
 

Requested From: United States
Date Requested: 12/10/2016

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