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Anti-SERPINH1 / HSP47 Antibody IHC-plus™ LS-B2151

Note: This antibody replaces LS-C48476

Ordering

Wt. Vol. Conc. Price
50 µg - 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SERPINH1 / HSP47 Antibodies

Anti-SERPINH1 / HSP47 Antibody (aa406-417) LS-C15723
Rabbit Polyclonal to Human SERPINH1 / HSP47
Human
Western blot
Unconjugated
Anti-SERPINH1 / HSP47 Antibody IHC-plus™ LS-B2151
Rabbit Polyclonal to Human SERPINH1 / HSP47
Human, Mouse, Rat, Chicken
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-SERPINH1 / HSP47 Antibody (clone M16.10A1) IHC-plus™ LS-B3677
Mouse Monoclonal [clone M16.10A1] (IgG2b) to Rat SERPINH1 / HSP47
Rat, Human, Mouse, Guinea pig, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SERPINH1 / HSP47
Human, Mouse, Rat, Chicken
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated

Details

Human SERPINH1 / HSP47
Rabbit
Human, Mouse, Rat, Chicken (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • IHC - Frozen (10 - 20 µg/ml)
  • Western blot (0.5 - 4 µg/ml)
  • Immunoprecipitation (10 - 20 µg/ml)

Specificity and Use

SERPINH1 / HSP47 antibody was raised against synthetic peptide surrounding amino acid 407 of human HSP47.
The antibody recognizes 47 kD Hsp47 of human, mouse, rat, and chick origins. Mouse small intestine tissue lysate can be used as a positive control.
Immunohistochemistry: LS-B2151 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2151 was determined to be 2.5 ug/ml.

Packaging

PBS, 30% glycerol, 0.5% BSA, 0.01% Thimerosal.
Long term: -70°C; Short term: -20°C; Avoid freeze-thaw cycles.
For research use only.

About SERPINH1 / HSP47

P50454 NM_001235 NP_001226.2

SERPINH1 Antibody, 47 kDa heat shock protein Antibody, AsTP3 Antibody, Colligin Antibody, Colligin-1 Antibody, Collagen-binding protein Antibody, Colligin-2 Antibody, CBP1 Antibody, HSP47 Antibody, OI10 Antibody, Serpin H1 Antibody, SERPINH2 Antibody, CBP2 Antibody, Collagen-binding protein 1 Antibody, Colligen Antibody, gp46 Antibody, PIG14 Antibody, PPROM Antibody, RA-A47 Antibody

SERPINH1 / HSP47 is a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis.

Immunohistochemistry

Immunohistochemistry
Anti-SERPINH1 / HSP47 antibody IHC of human skin, fibroblasts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2151 concentration 5 ug/ml.

Western blot

Western blot
Western blot of SERPINH1 / HSP47 antibody LS-B2151.

Requested From: United States
Date Requested: 12/7/2016

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