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Anti-SERPINE1 / PAI-1 Antibody IHC-plus™ LS-B7483

Note: This antibody replaces LS-C104565
Catalog Size Price
LS-B7483-50 50 µg (1 mg/ml) Unavailable

Most Popular SERPINE1 / PAI-1 Antibodies

Anti-SERPINE1 / PAI-1 Antibody (aa31-80) LS-C81062
Rabbit Polyclonal (IgG) to Human SERPINE1 / PAI-1
Human, Monkey, Mouse, Rat, Bovine, Pig, Rabbit
Western blot
Unconjugated
Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SERPINE1 / PAI-1 Antibody (clone 3 PAI) LS-C85375
Mouse Monoclonal [clone 3 PAI] (IgG2b) to Human SERPINE1 / PAI-1
Human
ELISA
Unconjugated
Anti-SERPINE1 / PAI-1 Antibody (aa110-145) LS-C91951
Mouse Monoclonal (IgG1) to Human SERPINE1 / PAI-1
Human
IHC, IHC - Frozen, Western blot, ELISA, Neutralization
Unconjugated
Anti-SERPINE1 / PAI-1 Antibody (aa71-85) IHC-plus™ LS-B3936
Goat Polyclonal to Human SERPINE1 / PAI-1
Human
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-SERPINE1 / PAI-1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3936 concentration 2.5 ug/ml.
Anti-SERPINE1 / PAI-1 Antibody IHC-plus™ LS-B7483
Rabbit Polyclonal (IgG) to Human SERPINE1 / PAI-1
Human
IHC, IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Anti-SERPINE1 / PAI-1 antibody IHC of human vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7483 concentration 5 ug/ml.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human SERPINE1 / PAI-1
Human
IHC, IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human SERPINE1 / PAI-1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA
  • Neutralization (15 µg/ml)

Specificity and Use

E.coli derived recombinant Human PAI-1.
Human PAI-1
Immunohistochemistry: LS-B7483 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPAI-1 (4.5 ug/ml), a concentration of at least 15 ug/ml of this antibody is required. ELISA: To detect hPAI-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PAI-1 (LS-C104564) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPAI-1. Western Blot: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About SERPINE1 / PAI-1

P05121 NM_000602 NP_000593.1

SERPINE1 Antibody, PAI1 Antibody, PLANH1 Antibody, PAI-1 Antibody, Serpin E1 Antibody

SERPINE1 / PAI-1 is a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

Immunohistochemistry

Anti-SERPINE1 / PAI-1 antibody IHC of human vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7483 concentration 5 ug/ml.
Anti-SERPINE1 / PAI-1 antibody IHC of human vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7483 concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of SERPINE1 / PAI-1 antibody LS-B7483
Western Blot (non-reducing) of SERPINE1 / PAI-1 antibody LS-B7483

Western blot

Western Blot (reducing) of SERPINE1 / PAI-1 antibody LS-B7483
Western Blot (reducing) of SERPINE1 / PAI-1 antibody LS-B7483

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of SERPINE1 / PAI-1 antibody LS-B7483
Sandwich ELISA of SERPINE1 / PAI-1 antibody LS-B7483

Requested From: 
Date Requested: 5/23/2018

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