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Catalog Number	Size	Price
LS-C59210-2	2 ml (90 mg/ml)	$1,225

SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha 1 anti-trypsin antibody-Fluorescent western blot. Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (LS-C59393, green); Alpha 1 anti-trypsin (Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody, red); and Human IgG (LS-C59376, Blue) in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Bio-Rad VersaDoc4000.

SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha-1anti-Trypsin Polyclonal Antibody-Western blot. Goat anti-Alpha-1anti-Trypsin antibody (LS-B1454 lot 5854) was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the Bio-Rad VersaDoc System.

SERPINA1 / Alpha 1 Antitrypsin Antibody - Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin (Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody lot 5842, red), Rabbit anti-Transferrin (LS-C59393 lot 3033), and Mouse-a-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the Bio-Rad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4?. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4?). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (lot 21100, shown as green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.

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Polyclonal Goat anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (IHC, WB) LS‑C59210

Name: Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody | Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C59210-2
Price: 1225.0000 USD
Availability: InStock

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Antibody:

SERPINA1 / Alpha 1 Antitrypsin Goat anti-Human Polyclonal Antibody

Application:

IHC, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$1,225

LS-C59210-2

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SERPINA1 / Alpha 1 Antitrypsin Goat anti-Human Polyclonal Antibody

Application:

IHC, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Alpha 1 Antitrypsin antibody LS-C59210 is an unconjugated goat polyclonal antibody to human Alpha 1 Antitrypsin (SERPINA1). Validated for ELISA, IHC and WB.

Target

Human SERPINA1 / Alpha 1 Antitrypsin

Synonyms

See All SERPINA1 / Alpha 1 Antitrypsin Antibodies

Host

Goat

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Delipidated and defibrinated

Modifications

Unmodified

Immunogen

A1-Anti-Trypsin [Human Plasma].

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified a1-Anti-Trypsin [Human Plasma]. Cross reactivity against a1-Anti-Trypsin from other sources is unknown.

Applications

IHC (1:500 - 1:2500)
Western blot (1:2000 - 1:10000)
ELISA (1:20000 - 1:100000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of ^1-Anti-Trypsin [Human Plasma] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) catalog no. LS-C60340 and ABTS as a substrate for 30 minutes at room temperature.

Presentation

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitution

Reconstitute in 2 mL of deionized water.

Storage

Store at 4°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SERPINA1 / Alpha 1 Antitrypsin