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Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody LS-C59210

Ordering

Wt. Vol. Conc. Price
- 2000 µl - $1,110
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SERPINA1 / Alpha 1 Antitrypsin Antibodies

Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody (C-Terminus) LS-C31768
Rabbit Polyclonal (IgG) to Human SERPINA1 / Alpha 1 Antitrypsin
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody IHC-plus™ LS-B3290
Goat Polyclonal (IgG) to Human SERPINA1 / Alpha 1 Antitrypsin
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human SERPINA1 / Alpha 1 Antitrypsin
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human SERPINA1 / Alpha 1 Antitrypsin
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • Western blot
  • Immunoprecipitation
  • ELISA (1:7000 - 1:30000)

Specificity and Use

SERPINA1 / Alpha 1 Antitrypsin antibody was raised against alpha-1-antitrypsin [Human Plasma].
Alpha-1-Anti-Trypsin [Human Plasma]. Cross reactivity against Alpha-1-Anti-Trypsin from other sources is unknown
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of ^1-Anti-Trypsin [Human Plasma] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) catalog no. LS-C60340 and ABTS as a substrate for 30 minutes at room temperature.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store at 4°C.
For research use only.

About SERPINA1 / Alpha 1 Antitrypsin

P01009 K02212 AAB59495.1

SERPINA1 Antibody, A1A Antibody, A1AT Antibody, AAT Antibody, Alpha 1 Antitrypsin Antibody, Alpha-1 protease inhibitor Antibody, Alpha-1-antiproteinase Antibody, Alpha-1-antitrypsin Antibody, Alpha1AT Antibody, PRO2275 Antibody, Alpha-1-antitrypsin null Antibody, PI1 Antibody, Serpin A1 Antibody

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin.

Western blot

Western blot
Alpha 1 anti-trypsin antibody-Fluorescent western blot. Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (LS-C59393, green); Alpha 1 anti-trypsin (LS-C59210, red); and Human IgG (LS-C59376, Blue) in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Bio-Rad VersaDoc4000.

Western blot

Western blot
Alpha-1anti-Trypsin Polyclonal Antibody-Western blot. Goat anti-Alpha-1anti-Trypsin antibody (LS-B1454 lot 5854) was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the Bio-Rad VersaDoc System.

Western blot

Western blot
Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin (LS-C59210 lot 5842, red), Rabbit anti-Transferrin (LS-C59393 lot 3033), and Mouse-a-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the Bio-Rad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (lot 21100, shown as green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.
 

Requested From: United States
Date Requested: 12/8/2016

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