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Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody IHC-plus™ LS-B421

Note: This antibody replaces LS-C18950
Catalog Size Price
LS-B421-50 50 µg (1 mg/ml) Unavailable

Most Popular SERPINA1 / Alpha 1 Antitrypsin Antibodies

Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody (C-Terminus) LS-C31768
Rabbit Polyclonal (IgG) to Human SERPINA1 / Alpha 1 Antitrypsin
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
SERPINA1 / Alpha-1-Antitrypsin antibody LS-C31768 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody IHC-plus™ LS-B421
Rabbit Polyclonal to Human SERPINA1 / Alpha 1 Antitrypsin
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B421 concentration 5 ug/ml.
Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody IHC-plus™ LS-B1454
Goat Polyclonal to Human SERPINA1 / Alpha 1 Antitrypsin
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1454 concentration 5 ug/ml.
Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody IHC-plus™ LS-B3290
Goat Polyclonal (IgG) to Human SERPINA1 / Alpha 1 Antitrypsin
Human
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Alpha-1-Antitrypsin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3290 concentration 5 ug/ml.
Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody (clone 15H10) IHC-plus™ LS-B10261
Mouse Monoclonal [clone 15H10] (IgG1) to Human SERPINA1 / Alpha 1 Antitrypsin
Human, Monkey, Dog
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10261 concentration 10 ug/ml.

100% Guaranteed
Rabbit Polyclonal to Human SERPINA1 / Alpha 1 Antitrypsin
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human SERPINA1 / Alpha 1 Antitrypsin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot
  • ELISA

Specificity and Use

Purified human Alpha-1-anti-Trypsin
Human Alpha-1-anti-Trypsin.
Immunohistochemistry: LS-B421 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B421 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.

About SERPINA1 / Alpha 1 Antitrypsin

P01009 K02212 AAB59495.1

SERPINA1 Antibody, A1A Antibody, A1AT Antibody, AAT Antibody, Alpha 1 Antitrypsin Antibody, Alpha-1 protease inhibitor Antibody, Alpha-1-antiproteinase Antibody, Alpha-1-antitrypsin Antibody, Alpha1AT Antibody, PRO2275 Antibody, Alpha-1-antitrypsin null Antibody, PI1 Antibody, Serpin A1 Antibody

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin.

Immunohistochemistry

Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B421 concentration 5 ug/ml.
Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B421 concentration 5 ug/ml.

Western blot

Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin (LS-C59210 lot 5842, red), Rabbit anti-Transferrin (LS-C59393 lot 3033), and Mouse-a-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (lot 21100, shown as green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.
Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin (LS-C59210 lot 5842, red), Rabbit anti-Transferrin (LS-C59393 lot 3033), and Mouse-a-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (lot 21100, shown as green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.

Requested From: 
Date Requested: 5/23/2018

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