Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-C355392-1	1 mg	$913

SERPINA1 / Alpha 1 Antitrypsin Antibody - Immunofluorescent analysis of Alpha1-Antitrypsin was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha1-Antitrypsin Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

SERPINA1 / Alpha 1 Antitrypsin Antibody - Western blot analysis was performed on membrane enriched extracts (30 ug) of (Fig 1) HT-29 (Lane 1) and Hep G2 (Lane 2). Likewise, Western blot analysis was performed on (Fig 2) condition media of Hep G2 (Lane 1).The blots were probed with Anti-Alpha1-Antitrypsin Mouse Monoclonal Antibody and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate.A~55 kDa band corresponding Alpha1-Antitrypsin was observed in HT-29, Hep G2 and in Hep G2 conditioned media. The observed shift in molecular weight from 50 kDa is due to glycosylation of the protein. Known quantity of protein samples were electrophoresed using4-12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody using iBind Flex Western Starter Kit.

1 of 2

2 of 2

Monoclonal Mouse anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (clone TMF1#4B5, IF, WB) LS‑C355392

Name: Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody | Mouse anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C355392-1
Price: 913.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

SERPINA1 / Alpha 1 Antitrypsin Mouse anti-Human Monoclonal (TMF1#4B5) Antibody

Application:

IF, WB, IP, ELISA, RIA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$913

LS-C355392-1

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SERPINA1 / Alpha 1 Antitrypsin Mouse anti-Human Monoclonal (TMF1#4B5) Antibody

Application:

IF, WB, IP, ELISA, RIA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Alpha 1 Antitrypsin antibody LS-C355392 is an unconjugated mouse monoclonal antibody to human Alpha 1 Antitrypsin (SERPINA1). Validated for ELISA, IF, IP, RIA and WB.

Target

Human SERPINA1 / Alpha 1 Antitrypsin

Synonyms

See All SERPINA1 / Alpha 1 Antitrypsin Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG2a Monoclonal

Clone

TMF1#4B5

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Immunogen

Purified human a1-Antitrypsin.

Specificity

The antibody targets Alpha1-Antitrypsin in IP applications and shows reactivity with Human samples. It detects Alpha1-Antitrypsin which has a predicted molecular weight of approximately 46 kD.

Applications